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The confocal configurations have been as follows: excitation 488 nm, emission 51030 nm, frame 5126512. A few-dimensional (3D) scanning was performed with a 3-mm Z-series venture phase, and 3D reconstructed photos of cells were used to determine relative fluorescence

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Right away following NaCl addition, for the duration of the following observation interval (three...
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The composition of the two lengthy helices, and the loop between them, is incredibly comparable to earlier J domain structures, but in P58IPK the two small helices are scarcely current, with each and every represented by just one particular flip of a helix

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Composite omit maps exhibiting the electron density corresponding to every of the domains and...