A 963.9 8.7 b 544.1 41.7 a increases in2 leaf dry weight compared to R1 plants, accompanied by a decrease in leaf area SLA (cm g-1DW) 557.7 three.eight c 433.1 three.2 b 276.7 1.1 a and, consequently, by a decrease in SLA (Table 1). 2.two.2. Effects of Prolonged Recovery after Very first Drought and Recovery right after Third 2.2.two. Effects of Prolonged Recovery immediately after Initial Drought and Recovery right after Third Drought Drought on Composition in WT and WT on Cell Wall Cell Wall Composition inflacca and Flaccaflacca Figure 7 Overlaid WT and flacca tomato leaves’ cell walls’ FTIR spectra are shown in Figure 7 for the handle plants, thethe plants following prolonged recovery along with the following 3 drought the control plants, plants soon after prolonged recovery and the plants plants following three cycles within the region of 800800 cm-1 . The cm-1. The bands cellulose, for example symmetric drought cycles in the region of 800800 bands associated to associated to cellulose, for instance CH2 vibration atvibration-at 1436 bending vibration at 1370 cm-1 , 1370 cm-1, CH2vibration symmetric CH2 1436 cm 1 , CH2 cm-1, CH2 bending vibration at CH2 wagging wagging at 1317 cm-11317 cm-1asymmetric stretchingstretching glycosidic link at 1151 cmat1 1151 vibration at , O , O asymmetric glycosidic link vibration vibration – and C and C stretching vibration at-1 had been identified in all cell wall YC-001 Description samples. The bands cm-1 stretching vibration at 1105 cm 1105 cm-1 have been found in all cell wall samples. The -1 – at 1735at 1735 (C=O (C=O stretching vibration of alkyl ester), 1635 1cm-1 (COOantisymbands cm cm-1 stretching vibration of alkyl ester), 1635 cm (COOantisymmet-1-1(COO stretching, acids), ric stretching vibration of polygalacturonic acid), 1420 cm metric stretching vibration of polygalacturonic acid), 1420 cm (COO stretching, 1 1240 cm-1 (C stretching) are characteristic for pectin, while the band at 1517 cm–1 is 1240 cm-1 (C stretching) are characteristic for pectin, even though the band at 1517 cm is -1 (O asymmetric stretching glycosidic characteristic for lignin. The bands at 1147 cm -1 (O asymmetric stretching glycosidic characteristic for lignin. The bands at 1147 cm hyperlink) and 1071 cm-1 (C stretching, C stretching) are typical for xyloglucan, as well as the hyperlink) and 1071 cm-1 (C stretching, C stretching) are standard for xyloglucan, along with the band at 896 cm-1is associated to cellulose, hemicellulose and pectin. band at 896 cm-1 is related to cellulose, hemicellulose and pectin.Figure 7. FTIR spectra from the cell walls ofof WT (A) andtomato(B) tomato leavesof the experiment. FTIR spectra from the cell walls WT and flacca flacca PHA-543613 Purity & Documentation leaves at the end at the end on the R1 represents represents plants the 1stdrought cycle and cycle and then watered for the for 15 experiment. R1plants exposed to exposed to the 1st drought then optimallyoptimally waterednextthe rd days. R days. R represents 3-days re-watered plants right after the 3rd cycle, whilst C represents respecnext 15 3 represents 3-days re-watered plants after the three droughtdrought cycle, whilst C represents three tive handle plants. respective manage plants.The effect of prolonged recovery, and/or 3 drought cycles on the content in the impact of prolonged recovery, and/or three drought cycles on the content of specific elements with the cell wall in the WT and flacca mutant leaves of tomato obtained certain elements on the cell wall in the WT and flaccamutant leaves of tomato obtained from the FTIR spectroscopy was analyzed employing principal component analyses (PCA). from the.
