benefits, obtained by solution ion scan mode analysis, had been observed from the product ion spectra obtained after the isolation of m/z 227.0 on Q1. This Caspase 9 Activator drug precursor ion could be the solution ion spectra obtained following the isolation of m/z 227.0 on Q1. This precursor ion is most likely represent the molecular ion CDK7 Inhibitor Storage & Stability ofionallegedalleged metabolite of 5, by de-nitration of probably to to represent the molecular an of an metabolite of five, obtained obtained by denitration with the side chain. Figure 9a reports the comparison on the m/z 227.0 chromatographic profiles obtained in the rat liver microsomal fraction prior to the incubation with compound five (dotted line) and immediately after two hours’ incubation (continuous line). A chromatographic peak is evident at the retention time of two.60 min only in theAntioxidants 2022, 11,12 ofthe side chain. Figure 9A reports the comparison on the m/z 227.0 chromatographic profiles obtained in the rat liver microsomal fraction just before the incubation with compound five (dotted line) and soon after two hours’ incubation (continuous line). A chromatographic peak is evident at the retention time of two.60 min only in the second profile, viz. after two hours’ incubation. The corresponding solution ion spectrum, depicted in Figure 9B, exhibits the Antioxidants 2022, ten, x FOR PEER Critique 13 of 21 loss of consecutive fragments in the side chain and it truly is compatible together with the supposed metabolite’s structure.Figure 9. (a) Superimposed mass chromatograms of thethe m/z 227.0 precursor ion, obtained from Figure 9. (A) Superimposed mass chromatograms of m/z 227.0 precursor ion, obtained from the rat liverliver microsomal fractiont at t0=(dotted line) and t = = two h (continuous line)incubation with all the rat microsomal fraction at = 0 (dotted line) and t 2 h (continuous line) incubation with compound 5. (b) Product ion spectrum from the selected m/z 227.0 precursor, collected at two.60 min, compound 5. (B) Solution ion spectrum in the chosen m/z 227.0 precursor, collected at two.60 min, in the latter evaluation. in the latter evaluation.Analogue experiments have been executed on the rat liver microsomal fraction incubated Analogue experiments were executed on the rat liver microsomal fraction incubated with compound 7. The m/z 288.0 precursor ion isolated on Q1 corresponds to the molecular with compound 7. The m/z 288.0 precursor ion isolated on Q1 corresponds to the molecularalleged metabolite 7 metabolite 7 obtained right after single the side chain.with the side ion with the ion from the alleged obtained following single de-nitration of de-nitration Figure 10A chain. Figure 10a reports the m/z 288.0 chromatographic profiles obtained from the rat reports the comparison of your comparison on the m/z 288.0 chromatographic profiles obtained from the fraction prior to the incubationbefore compound 7 and after two hours, liver microsomal rat liver microsomal fraction with the incubation with compound 7 and soon after two This time, a chromatographic peak is evident in the retention time of 3.78 respectively. hours, respectively. This time, a chromatographic peak is evident at the retention time of three.78 min only rat the profile from the rat liver microsomal fraction min only inside the profile from the in liver microsomal fraction collected immediately after two hours’ collected soon after two hours’ incubation. The ion spectrum, depicted ion Figure 10B, exhibits incubation. The corresponding solution corresponding item in spectrum, depicted in Figure 10b, exhibits a fragmentation related to Figure 9b. The item ion spe
