Product Name :
Mca-Lys-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 (fluorogenic substrate)
Sequence:
Mca-Lys-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2·TFA [Mca=(7-methoxycoumarin-4-yl)acetyl; Dpa=N-3-(2,4-dinitrophenyl)-L-α,β-diaminopropionyl]
Purity:
≥98% (HPLC)
Molecular Weight:
1221.3 . 114.0
Solubility :
Soluble in water (10mg/ml).
Appearance:
Lyophilized yellow solid.
Use/Stability :
As indicated on product label or CoA when stored as recommended.
Description:
MMP substrate Sensitive fluorogenic substrate for most matrix metalloproteinase (MMP) enzymes, as follows [kcat/Km (M-1s-1) and Km (µM), in parentheses]: MMP-1 (9.2 x 104, 27); MMP-2 (6.9×105, >30); MMP-3 (2.0×104); MMP-7 (2.9×105); MMP-8 (4.2×105); MMP-9 (6.2×105, >30); MMP-12 (1.3×105, 130); MMP-13 (1.1×106, 5.2); MMP-14 (1.3×106, 7.9); mouse, rat, and monkey MMP-14; MMP-16; MMP-20; ADAM10 (1.{{1970972-74-7} medchemexpress|{1970972-74-7} Biological Activity|{1970972-74-7} In Vitro|{1970972-74-7} custom synthesis} 1×103); ADAM17/TACE (7.8×105, 26), and BACE2 (internal data using Prod. No. BML-SE550). It is cleaved very poorly by neutrophil elastase, and not at all by trypsin. Can be used to measure cell-associated activity, or activity in cell-conditioned medium, or in biological fluids such as synovial fluid. Mca fluorescence is quenched by the Dpa group until cleavage separates them (MMPs cleave between Gly-Leu). Ex.: 328nm, Em.: 400nm, but it is possible to use Ex./Em. 320-340/393-420nm. This highly-quenched (98.{{161401-82-7} MedChemExpress|{161401-82-7} Technical Information|{161401-82-7} In stock|{161401-82-7} manufacturer} 4%) substrate is useful for inhibitor screening and kinetic analysis.PMID:27336130
CAS :
720710-69-0
Solubility:
Soluble in water (10mg/ml).
Formula:
C55H80N16O16 . CF3CO2H
Additional Information :
| Alternative Name FS-6 | Appearance Lyophilized yellow solid. | CAS 720710-69-0 | Formula C55H80N16O16 . CF3CO2H | MW 1221.3 . 114.0 | Purity ≥98% (HPLC) | Sequence Mca-Lys-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2·TFA [Mca=(7-methoxycoumarin-4-yl)acetyl; Dpa=N-3-(2,4-dinitrophenyl)-L-α,β-diaminopropionyl] | Solubility Soluble in water (10mg/ml). | Technical Info / Product Notes Also available: OMNIMMP® fluorogenic control peptide Mca-Pro-Leu-OH .
