Product Name :
Anti-Mouse IgG(Fcγ Fragment specific), AlpSdAbs® VHH(iFluor647)
Applications:
WB,ICC/IF,ELISA,Flow Cyt
Reactivity :
Mouse IgG(Fcγ Fragment specific)
Conjugate:
iFluor647
Advantages :
High lot-to-lot consistencyIncreased sensitivity and higher affinityAnimal-free production
Description:
| Description: Anti-Mouse IgG(Fcγ Fragment specific), AlpSdAbs® VHH(iFluor647) is designed for detecting mouse IgG Fcγ fragment(including mouse IgG1, IgG2a, IgG2b, IgG3) specifically, and Anti-Mouse IgG(Fcγ Fragment specific), AlpSdAbs® VHH(iFluor647) is useful for super-resolution microscopy. Anti-Mouse IgG(Fcγ Fragment specific), AlpSdAbs® VHH(iFluor647) is based on recombinant single domain antibody to mouse IgG Fc coupled to iFluor647. Based on immunoelectrophoresis and/or ELISA, Anti-Mouse IgG(Fcγ Fragment specific), AlpSdAbs® VHH(iFluor647) reacts with the Fc fragment of mouse IgG heavy chain but not with the Fab portion of mouse IgG. | Immunogen: Recombinant mouse IgG | Host: Alpaca pacous | Isotype: VHH domain of alpaca IgG2b/2c | Conjugate: iFluor647(Ex:651nm, Em:667nm) | Specificity: Mouse IgG(Fcγ fragment specific) | Cross-Reactivity: No cross-reactivity with mouse IgM, rabbit, human, cynomolgus, rat, goat IgG | Purity: Recombinant Expression and Affinity purified | Concentration: 0.5mg/mL | Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% glycerol | Storage: Store at –20 °C(Avoid freeze / thaw cycles), Protect from light. | Background: VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(The smaller size of the VHH decreases linkage error and increases staining accuracy effectively. Standard immunodetection approaches use typically a primary antibody (1.Ab) which binds the protein of interest (POI) and a secondary antibody (2.Ab) that binds to the 1.Ab and carries a detection element.{{2098191-53-6} site|{2098191-53-6} Biological Activity|{2098191-53-6} Description|{2098191-53-6} custom synthesis} The complex formed by the primary antibody and the secondary antibody (1.{{941987-60-6} site|{941987-60-6} Technical Information|{941987-60-6} References|{941987-60-6} manufacturer} Ab–2.PMID:29261901 Ab) is widely used because it is a cost effective and flexible approach since only the 2.Abs need to be coupled to the detection element. However, the use of this complex carries some relevant limitations. The 1.Ab–2.Ab can measure up to 30 nm, leading to a large distance between the targeted molecule and the detection element, causing the so called “linkage” or “displacement” error. While this might not influence the results in some applications (e.g. epifluorescence, ELISA or FACS), it is of major relevance for super-resolution microscopy techniques where the localization precision can be as high as 1 nm. The linkage error can be reduced by using directly labelled small affinity probes like camelid single domain antibodies (sdAbs) also known as nanobodies (Nbs), which have sizes below 3 nm.
Description2 :
Anti-Mouse IgG(Fcγ Fragment specific), AlpSdAbs® VHH(iFluor647) is designed for detecting mouse IgG Fcγ fragment(including mouse IgG1, IgG2a, IgG2b, IgG3) specifically, and Anti-Mouse IgG(Fcγ Fragment specific), AlpSdAbs® VHH(iFluor647) is useful for super-resolution microscopy. Anti-Mouse IgG(Fcγ Fragment specific), AlpSdAbs® VHH(iFluor647) is based on recombinant single domain antibody to mouse IgG Fc coupled to iFluor647. Based on immunoelectrophoresis and/or ELISA, Anti-Mouse IgG(Fcγ Fragment specific), AlpSdAbs® VHH(iFluor647) reacts with the Fc fragment of mouse IgG heavy chain but not with the Fab portion of mouse IgG.
Immunogen:
Recombinant mouse IgG
Host :
Alpaca pacous
Isotype:
VHH domain of alpaca IgG2b/2c
Purity :
Recombinant Expression and Affinity purified
Buffer :
Storage :
Store at –20 °C(Avoid freeze / thaw cycles), Protect from light.
Function:
Flow Cyt: 1:200-1:2000ICC/IF： 1:200-1:2000ELISA： 1:10000-1:50000WB: 1:10000-1:50000 | Super-resolution microscopyDilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
