Hhart D: Cytochromes p450. Arabidopsis Book 2012, 9:e0144.GUS staining was performed in accordance with a published approach [44].Metabolite profiling analysisWild form (W) and cyp709b3 (M) seedlings had been collected after two days (2D) and 4 days (4D) of development on typical or 150 mM NaCl MS plates. Sample extraction, LC/MS and GC/MS analysis had been performed as described [34] by METABOLON, Inc. In short, one hundred mg of each sample had been extracted making use of the automated Microlab STAR technique. Recovery standards were added prior to the very first step in the extraction process. Sample preparation was performed employing a proprietary series of organic and aqueous extractions to get rid of the protein fraction though allowing maximum recovery of small molecules. The resulting extract was divided into two fractions; a single for evaluation by LC/MS and one particular for analysis by GC/MS. Samples were placed briefly on a TurboVap(Zymark) to take away the organic solvent. Every sample was then frozen and dried beneath vacuum. Samples have been then ready for the suitable their instrument, either LC/ MS or GC/MS.Mao et al. BMC Plant Biology 2013, 13:169 http://www.biomedcentral/1471-2229/13/Page 13 of4. 5.six. 7.eight. 9.ten.11.12.13.14.15.16.17.18.19.20.21.22.23.24. 25.Durst F, Nelson DR: Diversity and evolution of plant P450 and P450reductases. Drug Metabol Drug Interact 1995, 12(three):18906. Paquette SM, Bak S, Feyereisen R: Intron-exon organization and phylogeny within a big superfamily, the paralogous cytochrome P450 genes of Arabidopsis thaliana. DNA Cell Biol 2000, 19(5):30717. Ralston L, Yu O: Metabolons involving plant cytochrome P450s. Phytochem Rev 2006, 5:45972. Schuler MA, Duan H, Bilgin M, Ali S: Arabidopsis cytochrome P450s by means of the hunting plass a window on plant biochemistry. Phytochem Rev 2006, 5:20537. Schuler MA, Werck-Reichhart D: Functional genomics of P450s. Annu Rev Plant Biol 2003, 54:62967. Narusaka Y, Narusaka M, Seki M, Umezawa T, Ishida J, Nakajima M, Enju A, Shinozaki K: Crosstalk within the responses to abiotic and biotic stresses in Arabidopsis: evaluation of gene expression in cytochrome P450 gene superfamily by cDNA microarray.2′-Deoxycytidine Biological Activity Plant Mol Biol 2004, 55(three):32742.PIPES References Ehlting J, Provart NJ, Werck-Reichhart D: Functional annotation from the Arabidopsis P450 superfamily depending on large-scale co-expression analysis.PMID:23776646 Biochem Soc Trans 2006, 34(Pt six):1192198. Ehlting J, Sauveplane V, Olry A, Ginglinger JF, Provart NJ, Werck-Reichhart D: An extensive (co-)expression evaluation tool for the cytochrome P450 superfamily in Arabidopsis thaliana. BMC Plant Biol 2008, 8:47. Matsuno M, Compagnon V, Schoch GA, Schmitt M, Debayle D, Bassard JE, Pollet B, Hehn A, Heintz D, Ullmann P, et al: Evolution of a novel phenolic pathway for pollen development. Science 2009, 325(5948):1688692. Godiard L, Sauviac L, Dalbin N, Liaubet L, Callard D, Czernic P, Marco Y: CYP76C2, an Arabidopsis thaliana cytochrome P450 gene expressed in the course of hypersensitive and developmental cell death. FEBS Lett 1998, 438(3):24549. Takei K, Yamaya T, Sakakibara H: Arabidopsis CYP735A1 and CYP735A2 encode cytokinin hydroxylases that catalyze the biosynthesis of trans-Zeatin. J Biol Chem 2004, 279(40):418661872. Kushiro T, Okamoto M, Nakabayashi K, Yamagishi K, Kitamura S, Asami T, Hirai N, Koshiba T, Kamiya Y, Nambara E: The Arabidopsis cytochrome P450 CYP707A encodes ABA 8-hydroxylases: important enzymes in ABA catabolism. EMBO J 2004, 23(7):1647656. Millar AA, Jacobsen JV, Ross JJ, Helliwell CA, Poole AT, Scofield G, Reid JB, Gubler F: Seed dormanc.
