Of stimulation (Fig. S3), except for TNF-a, where also monocytes are
Of stimulation (Fig. S3), except for TNF-a, where also monocytes are most likely to contribute for the TNF-a detected.—————————————————————————— “Figure 3. RhuDex1 impairs cytokine release of WO-LP and PB T cells. Cytokine concentrations had been measured in culture supernatants collected after 24 h of stimulation of WO-LPL, or LPS-activated PBMO co-cultured with non-adherent PBL. RhuDex1 and Abatacept have been added at the starting of culture. The imply cytokine responses of every donor inside the presence of inhibitors were normalized to the responses without inhibitors (medium, set to one hundred ). For (A) IL-17, (B) IFN-g, (C) IL-2, and (D) TNF-a, the upper graph of each panel depicts responses in WO-LPL (5 donors, numbered 1). The lower graph indicates responses in PBL of four allogeneic (allo, numbered I V) and three donors autologous (auto) to WO-LPL. Information points for every donor are shown in person colors, along with the imply SD of all information points in each condition is shown as columns and error bars. P 0.05; P 0.01; P 0.001; P 0.0001. Med, medium handle, Aba, Abatacept, Rhu, RhuDex1, inhibitor concentrations in mgmL.2014 The Authors. Immunity, Inflammation and Illness Published by John Wiley Sons Ltd.CD80 Blockage by RhuDex1 Reduces Intestinal T Cell IL-2 Protein supplier ActivationA.-K. IFN-beta, Human (HEK293) Heninger et al.Acytokine secretion [ ]250 200 150 one hundred 50 0 300IL-17 WO-LPL1 2 3 4IL-17 PBLI allo II allo III allo IV allo two auto three autocytokine secretion [ ]200 150 100 50 0 Med Aba ten Aba 1 Rhu 20 Rhu three Rhu 0.5 CD3 Med Aba ten Aba 1 Rhu 20 Rhu 3 Rhu 0.five CD4 auto five autoBcytokine secretion [ ]IFN- WO-LPL0IFN- PBLcytokine secretion [ ]0 Med Aba ten Aba 1 Rhu 20 Rhu three Rhu 0.5 CDFigure 3. Continued.Med Aba 10 Aba 1 Rhu 20 Rhu 3 Rhu 0.5 CD2014 The Authors. Immunity, Inflammation and Disease Published by John Wiley Sons Ltd.A.-K. Heninger et al.CD80 Blockage by RhuDex1 Reduces Intestinal T Cell ActivationCcytokine secretion [ ]250 200 150IL-2 WO-LPL1 two three 450 0IL-2 PBLI allo II allo III allocytokine secretion [ ]IV allo two auto three auto4 auto 5 auto0 Med Aba 10 Aba 1 Rhu 20 Rhu 3 Rhu 0.five Med Aba ten Aba 1 Rhu 20 Rhu three Rhu 0.five CD2 CD3Dcytokine secretion [ ]TNF- WO-LPL0 250 200 150 100 50 0 Med Aba ten Aba 1 Rhu 20 Rhu three Rhu 0.five CDFigure 3. Continued.2014 The Authors. Immunity, Inflammation and Illness Published by John Wiley Sons Ltd.TNF- PBLcytokine secretion [ ]Med Aba 10 Aba 1 Rhu 20 Rhu 3 Rhu 0.five CDCD80 Blockage by RhuDex1 Reduces Intestinal T Cell ActivationA.-K. Heninger et al.Figure four. Intracellular cytokine expression of CD4or CD8WO-LP and PB T cells after activation. (A) Proportion ( ) of CD4and CD8T cells amongst CD3T cells in PBL (3 allogeneic donors) and WO-LPL (two tissue donors). (B) Representative dot-plots (donor III) showing the intracellular cytokine expression (IL-17, IL-2, IFN-g and TNF-a) of CD4WO-LP T cells (left panel) and CD8WO-LP T cells (proper panel), or (C) of CD4PB T cells (left panel) and CD8PB T cells (ideal panel), as detected within the absence of stimulation, or six h of anti-CD3, or anti-CD2 stimulation.2014 The Authors. Immunity, Inflammation and Illness Published by John Wiley Sons Ltd.A.-K. Heninger et al.CD80 Blockage by RhuDex1 Reduces Intestinal T Cell ActivationAcytokine expression [ ]CD4 WO-LP T cells150 one hundred 50 0 150 one hundred 50 0 150 100 50 0 150 one hundred 50 0 TNFIFNIL-2 IL-Bcytokine expression [ ]CD4 PB T cells150 one hundred 50 0 150 100 50 0 150 one hundred 50 0 150 100 50 0 TNFIFNIL-2 IL-cytokine expression [ ]cytokine expression [ ]cytok.
