Of B18R Protein Biological Activity stimulation (Fig. S3), except for TNF-a, where also monocytes are
Of stimulation (Fig. S3), except for TNF-a, exactly where also monocytes are most likely to contribute for the TNF-a detected.—————————————————————————— “IL-2, Human Figure 3. RhuDex1 impairs cytokine release of WO-LP and PB T cells. Cytokine concentrations had been measured in culture supernatants collected right after 24 h of stimulation of WO-LPL, or LPS-activated PBMO co-cultured with non-adherent PBL. RhuDex1 and Abatacept had been added in the beginning of culture. The mean cytokine responses of each and every donor within the presence of inhibitors were normalized towards the responses devoid of inhibitors (medium, set to one hundred ). For (A) IL-17, (B) IFN-g, (C) IL-2, and (D) TNF-a, the upper graph of each and every panel depicts responses in WO-LPL (5 donors, numbered 1). The reduce graph indicates responses in PBL of 4 allogeneic (allo, numbered I V) and 3 donors autologous (auto) to WO-LPL. Data points for each donor are shown in individual colors, and also the imply SD of all data points in each condition is shown as columns and error bars. P 0.05; P 0.01; P 0.001; P 0.0001. Med, medium control, Aba, Abatacept, Rhu, RhuDex1, inhibitor concentrations in mgmL.2014 The Authors. Immunity, Inflammation and Disease Published by John Wiley Sons Ltd.CD80 Blockage by RhuDex1 Reduces Intestinal T Cell ActivationA.-K. Heninger et al.Acytokine secretion [ ]250 200 150 100 50 0 300IL-17 WO-LPL1 two 3 4IL-17 PBLI allo II allo III allo IV allo two auto three autocytokine secretion [ ]200 150 100 50 0 Med Aba 10 Aba 1 Rhu 20 Rhu three Rhu 0.5 CD3 Med Aba 10 Aba 1 Rhu 20 Rhu 3 Rhu 0.five CD4 auto 5 autoBcytokine secretion [ ]IFN- WO-LPL0IFN- PBLcytokine secretion [ ]0 Med Aba 10 Aba 1 Rhu 20 Rhu 3 Rhu 0.5 CDFigure three. Continued.Med Aba ten Aba 1 Rhu 20 Rhu 3 Rhu 0.five CD2014 The Authors. Immunity, Inflammation and Illness Published by John Wiley Sons Ltd.A.-K. Heninger et al.CD80 Blockage by RhuDex1 Reduces Intestinal T Cell ActivationCcytokine secretion [ ]250 200 150IL-2 WO-LPL1 two three 450 0IL-2 PBLI allo II allo III allocytokine secretion [ ]IV allo 2 auto three auto4 auto 5 auto0 Med Aba 10 Aba 1 Rhu 20 Rhu 3 Rhu 0.five Med Aba 10 Aba 1 Rhu 20 Rhu three Rhu 0.five CD2 CD3Dcytokine secretion [ ]TNF- WO-LPL0 250 200 150 one hundred 50 0 Med Aba ten Aba 1 Rhu 20 Rhu three Rhu 0.5 CDFigure 3. Continued.2014 The Authors. Immunity, Inflammation and Disease Published by John Wiley Sons Ltd.TNF- PBLcytokine secretion [ ]Med Aba ten Aba 1 Rhu 20 Rhu three Rhu 0.5 CDCD80 Blockage by RhuDex1 Reduces Intestinal T Cell ActivationA.-K. Heninger et al.Figure 4. Intracellular cytokine expression of CD4or CD8WO-LP and PB T cells after activation. (A) Proportion ( ) of CD4and CD8T cells amongst CD3T cells in PBL (three allogeneic donors) and WO-LPL (two tissue donors). (B) Representative dot-plots (donor III) displaying the intracellular cytokine expression (IL-17, IL-2, IFN-g and TNF-a) of CD4WO-LP T cells (left panel) and CD8WO-LP T cells (right panel), or (C) of CD4PB T cells (left panel) and CD8PB T cells (correct panel), as detected in the absence of stimulation, or six h of anti-CD3, or anti-CD2 stimulation.2014 The Authors. Immunity, Inflammation and Illness Published by John Wiley Sons Ltd.A.-K. Heninger et al.CD80 Blockage by RhuDex1 Reduces Intestinal T Cell ActivationAcytokine expression [ ]CD4 WO-LP T cells150 one hundred 50 0 150 one hundred 50 0 150 one hundred 50 0 150 100 50 0 TNFIFNIL-2 IL-Bcytokine expression [ ]CD4 PB T cells150 100 50 0 150 100 50 0 150 one hundred 50 0 150 100 50 0 TNFIFNIL-2 IL-cytokine expression [ ]cytokine expression [ ]cytok.
