Ome. It aids to reduce the symptoms of stomach and intestinal cramping. This medication works by slowing the all-natural movements on the gut and by relaxing the muscles inside the stomach and intestines. This combination is very effective and employed in the treatment of spasmodic dysmenorrhoea, intestinal colic, biliary colic, ureteric colic[3]. A literature survey relating to quantitative evaluation of those drugs revealed that attempts have already been created to create analytical approaches for the estimation of dicyclomine alone and in mixture with other drugs by liquid chromatographic technique [4], HPTLC CYP26 Inhibitor Synonyms methods[58] and spectrophotometric method[9]. For the estimation of mefenamic acid alone andNovember – DecemberIndian Journal of Pharmaceutical Sciencesijpsonlinein mixture with other drugs different liquid chromatographic methods[1014] and spectrophotometric methods[1521] procedures have already been reported. Distinctive analytical procedures have been reported for the estimation of paracetamol alone and in mixture with other drugs like spectrophotometry [2226] , liquid chromatography [2737] and HPTLC [3840] . An RPHPLC method[41] has lately been reported for the estimation of this drug combination. Present study requires improvement of a sensitive liquid chromatographic technique for the estimation of DIC, MEF and PCM in tablet dosage kind when compared with reported method.Preparation of regular stock options: DIC, MEF and PCM have been weighed (ten mg every) and transferred to 3 separate 10 ml volumetric flasks and dissolved in couple of milliliters of mobile phase. Volumes have been produced as much as the mark with mobile phase to yield a resolution containing 1000 /ml of each and every drug. Aliquot from the stock options of DIC, MEF and PCM have been appropriately diluted with mobile phase to receive working standard of one hundred /ml of DIC, MEF and PCM, respectively. Process validation: The approach was validated for accuracy, precision, linearity, detection limit, quantitation limit and robustness. Linearity was ascertained by taking suitable aliquots of DIC, MEF and PCM working normal options in different 10 ml volumetric flasks and diluted up to the mark with mobile phase to acquire final concentrations of ten, 30, 50, 70, one hundred /ml of DIC, 0.05, 0.25, 1, 5, 10 /ml of MEF, 0.1, 0.five, 2, 10, 20 /ml of PCM, respectively. The options had been injected using a 20 fixed loop system and chromatograms were recorded. Calibration curves were constructed by plotting average peak location versus concentrations and regression equations have been computed for each of the drugs. Repeatability research have been carried out by estimating response of DIC (50 /ml), MEF (1 /ml) and PCM (two /ml) six times and results are reported in terms of DYRK2 Inhibitor Biological Activity relative standard deviation. The intraday and interday precision research (intermediate precision) have been carried out by estimating the corresponding responses three occasions on the same day and on three different days for three distinct concentrations of DIC (30, 50, 100 /ml), MEF (0.25, 1, 10 /ml) and PCM (0.5, two, 20 /ml) along with the outcomes are reported when it comes to relative common deviation. Accuracy from the created technique was determined by system of standard additions. Recognized amount of DIC (0, 15, 30, 45 /ml), MEF (0, 1.25, 2.5, 5 /ml) and PCM (0, 2.five, 5, 7.5 /ml) had been added to a pre quantified sample remedy, along with the amount of DIC, MEF and PCM had been estimated by measuring the peak locations and by fitting these values towards the straightline equation of calibration curve. The limit of detection (LOD) is.
