Rmaceutical factories and medicinal herb growers attempted to boost market supply
Rmaceutical factories and medicinal herb growers attempted to improve market place provide by largescale planting, however the shortage of seedlings had limited the improvement of S. tonkinensis cultivation. Because 2008, we started to try and make S. tonkinensis plantlets through in vitro Tissue culture, and as much as now, we had developed one million tissue culture plantlets, which can meet 4000 mu (about 660 acres) planting necessity. As a result of our practice, we received a conclusion that tissue culture may be the most effective method to supply S. tonkinensis seedlings for agricultural cultivation. The form and concentration of phytohormones in medium had been vital from tissue culture materials propagation and rooting. In our investigation, we used BAP, KT, and IAA for bettering propagation, NAA, IBA, and ABT for rooting induction. BAP is surely an significant plant cytokinin, which can stimulate cell division, lateral bud emergence, and basal shoot formation.[18] KT (N6-furfuryladenine) wasPharmacognosy Magazine | October-December 2013 | Vol 9 | IssueKun-Hua, et al.: Tissue culture of Sophora tonkinensis GapnepACKNOWLEDGMENTThis review was supported from the Guangxi Purely natural Science Foundation of China (0991025Z), and Chinese herbal medicine support fund of National Advancement and Reform PIM1 custom synthesis Commission of China (2007-32).standing and top quality standard in Sophora tonkinensis. Da Zhong Ke Ji 2011;5:145-6. 13. Zhou YQ, Tan XM, Wu QH, Ling ZZ, Yu LY. A survey of unique plant of radix et rhizoma Sophora tonkinensis in Guangxi. Guangxi Sci 2010;17:259-62. 14. Qin LY, Tang MQ, Huang YC, Lin Y, Miao JH, Jiang N. The impact of storage temperature and time on seed vitality of Sophora tonkinensis. China Seed Indus 2011;1:35-6. 15. Gao SL, Zhu DN, Cai ZH, Xu DR. Autotetraploid plants from colchicine-treated bud culture of Salvia miltiorrhiza Bge. Plant Cell Tissue Organ Cult 1996;47:73-7. 16. Yao ShC, Ling ZhZh, Lan ZZ, Ma XJ. Optimization of tissue culture on Sophora tonkinensis Gapnep. Northern Hort 2011;six:136-9. 17. Murashige T, Skoog F. A revised medium for fast growth and bioassays with tobacco tissues cultures. Physiol Plant 1962;15:473-9. 18. Polanco MC, Pel z MI, Ruiz ML. Variables affecting callus and shoot formation from in vitro cultures of Lens culinaris Medik. Plant Cell Tissue Organ Cult 1988;15:175-82. 19. Miller CO, Skoog F, Saltza von MH, Powerful FM. Kinetin, a cell division component from deoxyribonucleic acid. J Am Chem Soc 1955;77:1392. 20. Miller CO, Skoog F, Okumura FS, Saltza von MH, Strong FM. Isolation, structure and synthesis of kinetin, a substrate promoting cell division. J Am Chem Soc 1956;78:1375-80. 21. Hagen G, Guilfoyle T. Auxin-responsive gene expression: Genes, promoters and regulatory variables. Plant Mol Biol 2002;49: 373-85. 22. Shen WH, Liu J, Tang QL. Examination on leaf traits and photosynthetic parameters of Eucalyptus clones. China Sci Tech 2010;24:69-71. 23. Kun-Hua W, Jian-Hua M, He-Ping H, Shan-Lin G. Generation of autotetraploid plant of ginger (Zingiber officinale Rosc.) and its high-quality evaluation. Pharmacogn Mag 2011;7:200-6.
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