He most enriched. A cell wall and an external encapsulating structure were detected within the cellular elements. For the KEGG enrichment analysis, mostidentified Aminopeptidase web pathways have been enriched in metabolism (Figure 4C). Only sesquiterpenoid and triterpenoid biosynthesis, steroid biosynthesis, monoterpenoid biosynthesis, cIAP1 Formulation nitrogen metabolism, and phenylpropanoid biosynthesis pathways were identified with important enrichment levels (Figure 4D). The outcomes in the GO and KEGG evaluation indicate that terpenoid-, steroid- and phenylpropanoid-related pathways might play important roles in TH growth.Identification of Candidate Genes for THTo further fully grasp the genetic mechanism of Salix matsudana Koidz. on fast-growing traits, we co-located the DEGs and 11 TH QTL on the reference genome. Our hypothesis is the fact that only these differentially expressed genes which can be situated in QTL regions may well be genetically associated with TH.Frontiers in Genetics | www.frontiersin.orgMarch 2021 | Volume 12 | ArticleLiu et al.Identify of Salix Height GenesTABLE three | Detailed details of 29 QTL for fast-growing in F1 population. Traits DBH QTL qDBH-A02-1 qDBH-B02-1 qDBH-B04-1 Atmosphere 2018 Combine Combine 2018 2019 Combine qDBH-B08-1 qDBH-B10-1 qDBH-A13-1 qDBH-A15-1 qDBH-A16-1 qDBH-B18-1 qDBH-B19-1 PCA qPCA-A01-1 qPCA-B02-1 qPCA-B04-1 qPCA-B08-1 qPCA-A10-1 qPCA-A11-1 qPCA-A15-1 qPCA-A16-1 TH qTH-A02-1 qTH-B02-1 qTH-A03-1 qTH-B04-1 qTH-A10-1 qTH-A11-1 2018 Combine 2018 Combine 2019 Combine 2018 Combine 2019 2018 2019 PCA PCA PCA PCA PCA PCA PCA PCA 2020 Combine 2018 Combine 2018 Combine 2018 Combine 2018 2020 Combine qTH-B13-1 qTH-A15-1 2018 2020 2018 2020 Combine qTH-A16-1 qTH-B16-1 qTH-B18-1 Combine 2018 2019 2020 Chromosome A2 A2 B2 B4 B4 B4 B8 B8 B10 B10 A13 A15 A16 A16 B18 B19 B19 A2 B2 B4 B8 A10 A11 A15 A16 A2 A2 B2 B2 A3 A3 B4 A10 A11 A11 A11 B13 B13 A15 A15 A15 A16 B16 B18 B18 LOD 3.62 3.48 two.five 3.37 two.75 three.97 three.67 three.31 2.94 2.74 2.73 2.73 two.73 two.5 2.78 two.58 2.72 3.02 2.51 3.24 two.92 2.52 two.94 2.73 two.51 four.06 2.65 2.61 two.57 2.66 2.67 three.02 three.13 2.82 three.37 3.23 2.66 three.08 three.01 three.54 2.9 two.59 2.83 two.85 3.82 R2 ( ) 9 eight.87 5.8 7.7 7.3 9.three 8.six 7.eight six.eight six.3 8.five six.three 7.7 five.9 7.8 5.9 six.9 7.two five.eight 7.7 6.9 6.9 7.3 six.9 five.9 ten 6.two 6 5.9 six.six 6.7 7.9 7.7 7 8.four 7.9 six.two 7.7 7.7 8.eight 6.7 6.two six.5 7.four 9.five 95 self-confidence interval (cM) 67.84-84.53 67.84-85.09 55.40-55.40 61.58-73.34 65.58-69.48 60.58-73.34 72.78-75.78 73.78-75.28 six.53-11.ten 7.53-10.ten 126.54-127.54 108.92-110.42 0-8.91 0.15-1.57 118.00-120.45 71.05-78.29 78.06-87.63 68.00-80.13 55.37-55.40 62.58-72.71 73.78-75.28 38.99-42.52 97.62-103.25 89.97-109.42 0.15-1.57 66.46-77.58 71.97-74.28 53.82-55.88 55.37-55.40 102.79-105.15 102.79-105.15 63.58-72.71 33.37-42.52 98.07-119.51 98.27-105.57 95.62-104.90 106.14-114.08 105.34-117.11 89.97-104.17 95.71-103.07 106.14-109.42 0.15-1.57 124.90-128.80 118.00-121.26 112.78-120.12 Physical interval (Mb) 7.11-8.41 7.11-8.69 three.86-4.00 5.80-6.32 5.85-6.05 five.78-6.32 5.24-5.76 5.24-5.76 0.39-0.96 0.74-0.96 11.45-11.50 ten.68-10.70 0.15-1.57 1.10-1.12 9.29-9.91 7.83-8.12 8.12-8.72 7.11-7.79 three.86-4.06 5.82-6.02 five.24-5.76 four.10-4.44 ten.35-10.55 9.46-10.70 1.10-1.12 7.09-7.67 7.26-7.46 three.80-4.06 3.86-4.06 eight.91-9.03 eight.91-9.03 5.83-6.02 three.42-4.44 10.36-11.50 10.36-11.04 10.34-10.70 10.53-10.82 ten.53-10.82 9.46-10.32 9.79-10.27 ten.53-10.70 1.10-1.57 14.11-14.45 9.29-10.06 8.98-9.Based on the reference genome, 228 from the 323 DEGs have been mapped on 38 chromosomes and 95 in the 323 DEGs have been mapped on scaffolds.