T two time points (LE day and HE day). EVs have been purified and characterized by nanoparticle-tracking evaluation and flow cytometry, and utilized to stimulate main endothelial cells for 24 h. EVs derived from endothelium (CD105+) and activated endothelium (CD62e+) had been successively quantified. Benefits: To start with plasma EV concentration was higher in HE day than in LE day (23,498 106/mL versus 5835 106/mL; p = 0.01) for all of the subjects. In endothelial cells exposed to subjects’ EVs, we analysed the ratio between CD105+ and CD62e+ produced EVs. We observed an improved CD62E+/CD105+ ratio, suggestive of an improved endothelial activation, in cells treated with HE day-EVs. Right after BMI stratification, we observed that the effect was due to NW subjects (CD62e+/CD105 + = 3.38 vs 1.39; p 0.0001) whereas EVs developed from OW subjects were not capable to induce this activation. Summary/Conclusion: EVs appear to possess the possible to act as marker of PM susceptibility and as molecular mechanism inside the chain of events connecting PM exposure to endothelial alterations, often linked to exposure and overall health threat. Funding: This project received support in the EU Programme “Ideas” (ERC-2011-StG 282413), principal investigator Prof. Valentina Bollati.Saturday, 05 MayPS06.Exosomes from high glucose-treated mesangial cells trigger dysfunction of podocytes Antonio S. Novaes1; Raphael Felizardo2; Niels OS Camara2; Mirian BoimFederal University of S Paulo, S Paulo, Brazil; 2University of S Paulo, S Paulo, BrazilBackground: Understanding of how mesangial cells communicate with podocytes inside the diabetic atmosphere is essential for the development of new targets for the prevention and remedy of diabetic nephropathy (DN). The aim of this study was to investigate whether or not exosomes secreted by high glucose-treated (HG-Exos) mouse mesangial cells (MMC) are capable to induce dysfunction of normal podocytes. Strategies: MMC were cultured under typical (five mM) or high glucose concentration (30 mM) for 24 h. Exos secreted towards the culture medium have been purified by ultracentrifugation. The vesicles size/concentration ratio was determined by the particle tracking (NanoSigth) and their characterization was performed by the presence of markers CD63 and CD81 by Western blot. Podocytes in culture were stimulated by HGExos for 24 h. Podocytes makers (actinin IV, p-cadherin and synaptopodin) and profibrotic markers (desmin, TGF-1 and collagen IV) have been analysed by qPCR. HG stimulus induced a change inside the quantity, but not in the size of Exos released by MMC. Results: HG-Exos induced phenotypic transition of podocytes that underwent epithelial mesenchymal transition, demonstrated by a downregulation of actinin 4, p-cadherin, synaptopodin collectively with an upregulation of desmin and TGF-1. Summary/Conclusion: These outcomes demonstrated the paracrine communication via exosomes amongst MMC and podocytes, and suggest that higher glucose stimulus in MMC can modified podocytes function contributing to DN. Funding: This study was funded by FAPESP Funda o de Amparo Pesquisa do Estado de S Paulo.are overrepresented in AKT Serine/Threonine Kinase 1 (AKT1) Proteins Recombinant Proteins prefrail and frail subjects in comparison with non-frail (ANOVA test, p 0.01). Summary/Conclusion: The boost in CD3, CD4 and CD197 derived MVs in prefrail and frail men and women may very well be related towards the chronic lowgrade state of inflammation. The considerable presence of CD221+ derived MVs in prefrail and frail sufferers may very well be linked to IGFR, which can be ADAM17/TACE Proteins web already recognized as a prevalent b.
