Tion, wax, eggs and animal protein components, presumably inside the affresco approach. Additionally, wax, eggs and [80]. identified, the presence of which can be on account of prior restoration workanimal glue have already been identified, the presence of which can be on account of earlier restoration function [80].Figure 1. The picture of Season in space 42 (the antechamber of your so-called erotic cubiculum) of Figure 1. The picture of Season in space 42 (the antechamber of the so-called erotic cubiculum) of Insula del Centenario, Pompeii, Italy, where sample 42/2 was collected. Insula del Centenario, Pompeii, Italy, where sample 42/2 was collected.A paper has been published on Villa from the Papyri in Herculaneum, so as to know the presence of an organic binder. The authors identified egg, by implies of GC-MS, in some samples, where the pigment essential a secco approach. They also investigated the presence of wax employed as superficial treatment options of the wall painting. The fact that they didn’t detect beeswax in all the analyzed samples give further data that the encaustic technique was not used for the realization from the wall paintings in the Vesuvian area [81]. An exciting work examined the Spiperone Biological Activity wooden roof and ceiling on the Property from the Telephus Relief in Herculaneum. The objective of this study was to assess the techniques utilised to decorate the wooden ceiling, working with a multianalytical strategy (SEM/EDX, FT-IR spectroscopy, GC/MS and Py-GC/MS). The outcomes showed that a tempera painting has been selected. Egg was employed as binding medium, as confirmed by the evaluation in the proteinaceous fraction and by the presence of cholesterol. In only a single sample, caseinAppl. Sci. 2021, 11,12 ofwas determined, probably employed as an adhesive. The paint layer was directly applied the wooden ceiling, without having a preparatory stratum. A motivating aspect of this paper is the fact that the authors argue that, primarily based on chemical investigations, that is the initial time it has been possible to prove that egg tempera has been employed to decorate wooden architectural elements [82]. Cunhighlights the truth that investigations of ancient Roman wall painting show the difficulty of effectively extracting organic binders. The author considers that from time to time it can be regarded an affresco painting, as a result of lack of benefits obtained, when the organic binder might be present, but not identified for purely analytical troubles. The usage of inappropriate extraction approaches for ancient paint supports can cause the transformation of some elements from the binder, creating an incomplete characterization with the composition He pointed out that chemical analyses of Roman wall painting have identified other varieties of paint IL-6 Protein Gene ID binders and that there’s a clear distinction among the results of a single investigation group along with the other. These factors may very well be associated for the difficulty of extracting ancient painting binders and, additionally, the influence of microbiological contamination. The absence of data in most studies on the efficiency of extraction actions utilised in antique samples plus the effect of microbial contamination may give rise to doubts about a few of the final results obtained [8]. Cunet al. (2012) studied eight samples of Roman wall paintings from 3 archaeological websites in Spain utilizing complete attenuated reflectance Fourier transform infrared spectroscopy and GC-MS, to be able to recognize organic binder. It was noticed the samples featured beeswax and soap. These final results show for the first time that Roman artists made use of in mural paintings.