Ts unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) RR6 web applies to the data made available in this article, unless otherwise stated.Tripathi et al. Standards in Genomic Sciences (2016) 11:Page 2 ofLampropedia cohaerens was sequenced in order to supplement the phenotypic taxonomical observations with genetic data and obtain genomic insights into heavy metal resistance and metabolic potential of gene complements of this microbial mat dweller. Here, we describe the summary classification, properties, genome sequencing, assembly and annotation of L. cohaerens CT6T (DSM 100029T=KCTC 42939T).Table 1 Classification and general features of Lampropedia cohaerens CT6T [39, 40]MIGS Id Property Classification Term Domain Bacteria Phylum Proteobacteria Class Betaproteobacteria Order Burkholderiales Family Comamonadaceae Genus Lampropedia Species cohaerens Type strain: Strain CT6T (Accession: DSM 100029T) Gram stain Cell-shape Motility Sporulation Temperature range Optimum temperature pH range; Optimum Negative Coccoid Non-motile Not reported 20?5 37 6? Evidence codea TAS [41] TAS [42, 43] TAS [44, 45] TAS [44, 46] TAS [1] TAS [2, 47] TAS [6] TAS [6] TAS [6] TAS [6] TAS [6] NAS TAS [6] TAS [6] TAS [6] TAS [6] TAS [6] TAS [6] TAS [6] NAS NAS TAS [6] IDA IDA IDA IDAOrganism informationClassification and featuresL. cohaerens was characterized by using a polyphasic approach with the integration of genotypic, phenotypic and chemotaxonomic methods [6]. This Gram-stain-negative, aerobic bacterial strain, forms white, smooth colonies with irregular margins on LB agar [6]. Transmission electron microscopy (TEM) revealed coccoid, unflagellated cells approximately 0.62 m ?0.39 m in dimension (Fig. 1). Summary characteristics are mentioned in Table 1. The slightly thermophilic and arsenic tolerant L. cohaerens strain CT6 can tolerate temperature in the range 20?5 and can tolerate arsenic trioxide up to 80 parts per billion [6]. The NaCl tolerance for strain CT6T was tested as 1? (w/v) and pH range as 6?. Biofilm formation PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/28381880 is observed in LB media, inspiring its etymology. L. cohaerens showed closest phylogenetic similarity to “L. puyangensis 2-binT” (96.4 ) and L. hyalina ATCC 11041T (95.4 ) on the basis of 16S rRNA gene sequences. A maximum-likelihood [14] phylogenetic tree based on Jukes-Cantor [15] model using MEGA version 6 [16] constructed with closely related members of family Comamonadaceae on the basis of Blast-n [17] of 16S rRNA gene placed strain CT6T along with the members of genus Lampropedia with bootstrap [18] confidence value of 98 (Fig. 2). Positive biochemical tests included theCarbon source Capric acid, Malic acid, Citric acid MIGS-6 Habitat Microbial mat 1? NaCl (w/v) Aerobic Free-living Non-pathogenic India 2014 31.378473 77.406945 1700 mMIGS-6.3 Salinity MIGS-22 Oxygen requirement MIGS-15 Biotic relationship MIGS-14 Pathogenicity MIGS-4 MIGS-5 Geographic Location Sample collectionMIGS-4.1 Latitude MIGS-4.2 Longitude MIGS-4.4 AltitudeaEvidence codes – IDA Inferred from Direct Assay, TAS Traceable Author Statement (i.e., a direct report exists in the literature), NAS Non-traceable Author Statement (i.e., not directly observed for the living, isolated sample,.
