Ions depending on the ways of classifying and grading gliomas, even between primary and secondary gliomas and between pediatric and adult gliomas [14,20,36,37]. Third, consistency of the experimental results at DNA, RNA, and protein levels is poor due to complications of Autophagy transcriptional and posttranslational regulations, as well as enzymatic modifications [6,12,38,39]. Fourth, relationship between sequence variations and prognosis are influenced by certain patient-specific parameters. For instance, older patients tend to have shorter survival time than younger patients [1,40]. Fifth, co-variations can occur in different chromosomes and one gene may have multiple variations in different combinations [41,42]. Last, relatively homogenous DNA variations have been found when topological locations (e.g., central vs. peripheral) are compared, where cross-contamination between normal 
and tumor tissues may contribute to the incorrect detection of structural variations [6,43,44]. Nevertheless, the existing literature related to Epigenetics genomic alterations and their molecular mechanisms 15481974 in gliomas lacks information from Chinese populations. Together with increasing throughput and efficiency of sequencing and microarray technologies for SNP and CNV discovery and typing, in-depth study on special tumor types become feasible,Gene AASSPrimer F: AGCGCTACATAAGTCGTT R: GTCACATATTGCCACGAGTTTProduct size (bp)Fold change 1.CYP2JF: AAAGAAGCCCTTATCCAC R: GAATGCGTTCCTCTAAGCTCT30.CYP4AF: CCTGGACCAGATGCCCTACAC R: CAGGGAAGGTGACGGGAGT111.PLA2G2AF: CATGATCTTTGGCCTACTGC R: CAGCCGTAGAAGCCATAACTG6.PLA2GF: ACATTCGCACACAGTCCTAC R: GAGGATGTTGGGAAAGTATTG24.PTENF: CAGAAAGACTTGAAGGCGTAT R: TTGGCGGTGTCATAATGT52.RBF: TCCTCGAAGCCCTTACAAGTT R: TCTCAGAAGTCCCGAATGAT6.Note: These primers were designed by using Oligo6. Fold changes were computed by comparing between HGG and LGG. doi:10.1371/journal.pone.0057168.tGenomic Aberration Patterns in GliomasTable 3. Comparison of sample numbers of genomic aberration in gliomas at the cytoband level.Homozygous deletionsL H8p11.23 13q12.11 5q14.3 6q12 6q14.2 6q21 6q22.32 6q23.2 6q24.2 13q12.3 13q14.12 13q14.3 13q21.31 19q13.12 2p12 6p22.1 22q12.3 2p23.2 3q26.1 6q25.1 9p24.1 10p15.1 17p13.3 18q22.3 22q13.31 1p13.3 1p22.2 1p31.3 1p32.3 1p35.1 1p36.11 1p36.31 5p15.33 7q31.33 7q35 7p11.2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 3 4 4 4 4 4 4 4 4 4 4 2 7q31.31 7q33 7q36.1 4 4 4 7q31.32 7q34 20q11.1 4 4 4 1p21.1 1p22.3 1p32.1 1p33 1p35.2 1p36.12 4 4 4 4 4 4 1p21.3 1p31.1 1p32.2 1p34.3 1p35.3 1p36.13 4 4 4 4 4 4 2p25.1 6p21.32 8q22.3 9p24.2 14q32.13 17q22 18q23 3 3 2 2 2 2 2 2p25.3 6p21.33 8q24.3 10p13 15q26.1 18q22.1 21q22.3 2 3 3 2 2 2 2 6p21.32 6q15 2 2 6p21.33 9p24.1 2 2 11q11 6q13 6q14.3 6q22.1 6q22.33 6q23.3 11p15.4 13q13.1 13q14.13 13q21.1 19q12 2 2 2 2 2 2 2 2 2 2 2 6q14.1 6q16.3 6q22.31 6q23.1 6q24.1 13q12.2 13q13.2 13q14.2 13q21.2 19q13.11 2 2 2 2 2 2 2 2 2GeneCoDis3 was used to relate genes to the corresponding Gene Ontology (GO) terms with cut-off = 0.05 [46], and WebGestalt V2 was used to map genes onto significant KEGG pathways with cut-off = 0.1 [47]. OMIM (Online Mendelian Inheritance in Man) was used to find relationship between genes and diseases [48].Hemizygous deletionsL H H H H H H H H H H HReal-time qPCR-based Validation ExperimentFour RNA samples, reverse-transcribed (Table 1), were subjected to real-time qPCR in a final reaction volume of 10 ml, which contains: 1 ml of 106buffer, 0.25 ml 
of dNTP, 2 ml of genespecific forward an.Ions depending on the ways of classifying and grading gliomas, even between primary and secondary gliomas and between pediatric and adult gliomas [14,20,36,37]. Third, consistency of the experimental results at DNA, RNA, and protein levels is poor due to complications of transcriptional and posttranslational regulations, as well as enzymatic modifications [6,12,38,39]. Fourth, relationship between sequence variations and prognosis are influenced by certain patient-specific parameters. For instance, older patients tend to have shorter survival time than younger patients [1,40]. Fifth, co-variations can occur in different chromosomes and one gene may have multiple variations in different combinations [41,42]. Last, relatively homogenous DNA variations have been found when topological locations (e.g., central vs. peripheral) are compared, where cross-contamination between normal and tumor tissues may contribute to the incorrect detection of structural variations [6,43,44]. Nevertheless, the existing literature related to genomic alterations and their molecular mechanisms 15481974 in gliomas lacks information from Chinese populations. Together with increasing throughput and efficiency of sequencing and microarray technologies for SNP and CNV discovery and typing, in-depth study on special tumor types become feasible,Gene AASSPrimer F: AGCGCTACATAAGTCGTT R: GTCACATATTGCCACGAGTTTProduct size (bp)Fold change 1.CYP2JF: AAAGAAGCCCTTATCCAC R: GAATGCGTTCCTCTAAGCTCT30.CYP4AF: CCTGGACCAGATGCCCTACAC R: CAGGGAAGGTGACGGGAGT111.PLA2G2AF: CATGATCTTTGGCCTACTGC R: CAGCCGTAGAAGCCATAACTG6.PLA2GF: ACATTCGCACACAGTCCTAC R: GAGGATGTTGGGAAAGTATTG24.PTENF: CAGAAAGACTTGAAGGCGTAT R: TTGGCGGTGTCATAATGT52.RBF: TCCTCGAAGCCCTTACAAGTT R: TCTCAGAAGTCCCGAATGAT6.Note: These primers were designed by using Oligo6. Fold changes were computed by comparing between HGG and LGG. doi:10.1371/journal.pone.0057168.tGenomic Aberration Patterns in GliomasTable 3. Comparison of sample numbers of genomic aberration in gliomas at the cytoband level.Homozygous deletionsL H8p11.23 13q12.11 5q14.3 6q12 6q14.2 6q21 6q22.32 6q23.2 6q24.2 13q12.3 13q14.12 13q14.3 13q21.31 19q13.12 2p12 6p22.1 22q12.3 2p23.2 3q26.1 6q25.1 9p24.1 10p15.1 17p13.3 18q22.3 22q13.31 1p13.3 1p22.2 1p31.3 1p32.3 1p35.1 1p36.11 1p36.31 5p15.33 7q31.33 7q35 7p11.2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 3 4 4 4 4 4 4 4 4 4 4 2 7q31.31 7q33 7q36.1 4 4 4 7q31.32 7q34 20q11.1 4 4 4 1p21.1 1p22.3 1p32.1 1p33 1p35.2 1p36.12 4 4 4 4 4 4 1p21.3 1p31.1 1p32.2 1p34.3 1p35.3 1p36.13 4 4 4 4 4 4 2p25.1 6p21.32 8q22.3 9p24.2 14q32.13 17q22 18q23 3 3 2 2 2 2 2 2p25.3 6p21.33 8q24.3 10p13 15q26.1 18q22.1 21q22.3 2 3 3 2 2 2 2 6p21.32 6q15 2 2 6p21.33 9p24.1 2 2 11q11 6q13 6q14.3 6q22.1 6q22.33 6q23.3 11p15.4 13q13.1 13q14.13 13q21.1 19q12 2 2 2 2 2 2 2 2 2 2 2 6q14.1 6q16.3 6q22.31 6q23.1 6q24.1 13q12.2 13q13.2 13q14.2 13q21.2 19q13.11 2 2 2 2 2 2 2 2 2GeneCoDis3 was used to relate genes to the corresponding Gene Ontology (GO) terms with cut-off = 0.05 [46], and WebGestalt V2 was used to map genes onto significant KEGG pathways with cut-off = 0.1 [47]. OMIM (Online Mendelian Inheritance in Man) was used to find relationship between genes and diseases [48].Hemizygous deletionsL H H H H H H H H H H HReal-time qPCR-based Validation ExperimentFour RNA samples, reverse-transcribed (Table 1), were subjected to real-time qPCR in a final reaction volume of 10 ml, which contains: 1 ml of 106buffer, 0.25 ml of dNTP, 2 ml of genespecific forward an.
