tions tends to lead to resistance to such viruses, possibly by avoidance of recognition. Our analysis is limited by the availability of only one sequenced E. huxleyi genome which is from a virus susceptible strain. Therefore we can not identify possible resistance genes in the resistant strains due to the limitation of our microarray design. We therefore regard it as very likely that virus susceptibility of E. huxleyi may be dependent on the expression of other genes or factors for viral entry. However, differences in copy numbers or pointmutations of coding sequences of the identified receptor-like protein kinases could be an indication for differences in virus susceptibility, making them suitable targets for further studies. and Mackinder et al. ) and the discovery of novel genes possibly involved in calcification and coccolithogenesis by using EST approaches, suppression subtractive hybridization, long serial analysis of gene expression, microarrays for gene expression analysis and quantitative RT-PCR, the details of the process of and the genes involved in coccolith formation in E. huxleyi are still unknown. Genes potentially involved in calcification like carbonic anhydrase or the calcium-binding glycoprotein with a high glutamic acid, proline, and alanine content were not detected as calcifying factors in our study. This indicates that these two genes might be regulated at the transcript level or they fulfill cell-biological tasks in the non-calcifying life-cycle stage as well, as also indicated recently by Dassow et al. and Rokitta et al.. Emiliania huxelyi is known for its flexible responses in ecophysiological studies. In particular, recent studies on carbonate chemistry changes showed strain-specific sensitivities to acidification of seawater which might be due to genetic variability described here. However, even with the same strain the diploid stage 1916 1685439 and the haploid 1917 exhibit different strategies and gene sets to acclimate to changing environmental conditions. The genes possibly involved in virus susceptibility and calcification identified in this study provide targets for future studies on their expression, e.g. under virus attack, and for gene knock-out experiments. Methods Strains and culture conditions Emiliania huxleyi strains and Gephyrocapsa oceanica were cultured in f/2 medium and Isochrysis galbana in K media at 15uC with a 16:8 light-dark cycle and 150 14522929 mE m22 s21. Strains EH2 and NZEH were treated with 1000 mg/mL Kanamycin because they were too sensitive against the antibiotic mixture. All other cultures were treated with a mixture of Ampicillin, Gentamycin, Streptomycin, Chloramphenicol and Ciprofloxacin. Antibiotic treatment took place over 1012 days. After 56 days cultures grown in 200 mL treated with antibiotics were transferred to 800 mL antibiotic treated f/2 media. Five to six days later 
cells were harvested on 1.2 mm RTTP ISOPORE filters Millipore. Cultures were checked against bacteria with acridine-orange staining. Only samples with no observed bacteria were used for analysis, although we cannot reduce a highly reduced bacterial background. Genes involved in calcification A total of only 11 genes were identified as possibly associated with calcification. Three of them showed no similarity to sequences in the public sequence databases or were of unknown GFT-505 web function. We identified a kelch-like protein, one activator of 90 kDa heat shock protein ATPase homolog, and one uncharacterized oxidoreductase. Mor
